Figure 3

FRAP suggests increased release of GFP-Gsα from lipid rafts after chronic antidepressant but not lithium treatment. a Typical course of photobleaching with representative images of cell before photobleaching (t = −3 s), immediately after photobleaching (t = 0 s), and after maximal recovery of fluorescence (t = 45 s). b Demonstration of typical fluorescence recovery after photobleaching in cells treated with escitalopram 10 µM or LiCl 3 mM for 72 h as described in “Methods”. Yellow arrows indicate area of bleach and recovery.