Skip to main content
Fig. 1 | BMC Neuroscience

Fig. 1

From: Scutellarin regulates microglia-mediated TNC1 astrocytic reaction and astrogliosis in cerebral ischemia in the adult rats

Fig. 1

Cell viability assay of TNC1 astrocytes (a): scutellarin (in the range of 0.2–2.0 mM) incubated for different duration did not result in any significant cell death. Treatment of TNC1 with scutellarin (b): scutellarin at 0.54 mM did not elicit a noticeable reaction in TNC1 whose GFAP/TNF-α (B13) expression remained comparable to cells in the control in basic medium (BM) (A13). BM + S, basic medium + scutellarin. Microglia mediate TNC1 astrocyte reaction (c): TNF-α mRNA expression in TNC1 astrocytes remained relatively unchanged at all time-points following treatment with BM, BM + L and BV-2 conditioned medium (CM). However, in TNC1 incubated CM + L for various time points, TNC1 showed a remarkable increase in TNF-α peaking at 24 h. Confocal images in d showing GFAP (C13), and TNF-α (D13) expression in TNC1 astrocytes incubated with different medium for 24 h. Compared with cells incubated in BM and BM + LPS (BM + L), TNC1 astrocytes incubated with LPS-stimulated BV-2 cell conditioned medium (CM + L) (C3, D3) are hypertrophic and exhibit a marked increase in GFAP and TNF-α immunofluorescence. Scale bars 20 μm

Back to article page