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Table 1 Primer sets for amplification of the open reading frame of zebrafish wdr81

From: Characterization of a novel zebrafish (Danio rerio) gene, wdr81, associated with cerebellar ataxia, mental retardation and dysequilibrium syndrome (CAMRQ)

 

Primer sequence

Annealing temperature (°C)

Expected amplicon size (bp)

Pair 1

 F

5′-GCAAACAGTGCAGAGCTTCTT-3′

60

897

 R

5′-GCTGCTCATCAACTGCAATATC-3′

Pair 2

 F

5′-CCTATCCACCTGCTCAGCTC-3′

60

874

 R

5′-AACATGGCTGCATAGCACAG-3′

Pair 3

 F

5′-CCAGATCTTGGTGGACCAGT-3′

60

909

 R

5′-TCTGTGAAGCATGGCAGTTC-3′

Pair 4

 F

5′-TTGGTTTGGTTGTGTCTCCA-3′

60

875

 R

5′-ATACCAGGCCGCATAAACAG-3′

Pair 5

 F

5′-CTCCATCATGCACTGGACAC-3′

60

920

 R

5′-CCAACTGTTGTTGCTCCAGA-3′

Pair 6

 F

5′-GCCACATCTTCTGGCAAAGT-3′

55

979

 R

5′-TGAGTACCACAGCACCCAAA -3′

Pair 7

 F

5′-GAGACCAGACTGCAAGACCAG-3′

55

698

 R

5′-ACTTGCTCGTTCGTGGTAAGA-3′

Pair 8

 F

5′-GCAGAGTGCACATACCTGGA-3′

60

704

 R

5′-TGGAAGTGGAAGTGGGAGTC-3′

Pair 9

 F

5′-AACAGGACCTTCCACGTAGC-3′

60

896

 R

5′-TGTGCTGTCCAGAATGGAGT-3′

Pair 10

 F

5′-CACAAGCCACTCCACCAGTA-3′

60

429

 R

5′-CGCGGAGGTTGTAAGTTCTC-3′