Table 3 Primer pairs used in the characterization of 3′ end of wdr81
Primer | Primer sequence | Expected amplicon size (bp) | |
|---|---|---|---|
Primer pair 1 | F (Pair1F_Drwdr81_3RACE) | 5′-CTGACAACGGTGCCATCAGG-3′ | 717 |
R (Pair1R_Drwdr81_3RACE) | 5′-TTCAGGACCATCCCATTGCATA-3′ | ||
Primer pair 2 | F (Pair2F_Drwdr81_3RACE) | 5′-CTGTATCCACGTCAATGGAGCGTAA-3′ | 757 |
R (Pair2R_Drwdr81_3RACE) | 5′-GAAGCATTGTTCAATGTACGTTCGGTA-3′ | ||
Primer pair 3 | F (Pair3F_Drwdr81_3RACE) | 5′-CATTTATGGTTCGCTAATTCCCTCAA-3′ | 634 |
R (GeneRacer™ 3′ Primer) | 5′-GCTGTCAACGATACGCTACGTAACG-3′ |