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Fig. 2 | BMC Neuroscience

Fig. 2

From: Intracellular chloride concentration of the mouse vomeronasal neuron

Fig. 2

Measurement of intracellular chloride concentration in VNO neuron. a Fluorescence signal of a VNO slice loaded with MQAE dye and incubated in ionophore cocktail in 15 mM (left) and 150 mM (right) standard solutions. Scale bar is 10 μm. b Repeated calibration using the two standard solutions indicates no obvious bleaching. Fluorescent intensity (arbitrary unit) is plotted over time. Top three bars indicate incubation with 15 mM solution and bottom two bars indicate incubation with 150 mM solution. ACSF is used before and after the calibration. c Stern–Volmer equation fit (black lines) to fluorescent signals using standard intracellular chloride concentration (black squares). Average intensities of MQAE fluorescence of VNO neurons at rest (Tyrode) and during urine application are extrapolated to obtain [Cl]in. Data is shown as mean ± s.e.m

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