Fig. 2

Survival and localization of ENSCs following intracerebral delivery. Nestin-GFP⁺ ENSCs were injected into various brain regions and mice were analyzed at 2 weeks and 4 weeks post transplantation into the brain regions depicted (A). Transplanted cells were found in clusters (B) and monolayers (C) along the ventricular lining, and within the parenchyma of the hippocampus formation (D, F–H) and subventricular zone (E). BrdU labeling confirms survival of proliferating ENSCs (E). Differentiation into glial (E) and neuronal (F–H) phenotypes is also seen (F represents an inset from the same region as G, with Tuj1 as a marker of mature neurons). Increased numbers of endogenous doublecortin⁺ neurons within the dentate gyrus are identified in close proximity to GFP⁺ transplanted cells (G, arrow)