Fig. 2
Temporal relationship of AQP1 expressions in spine cord after IR injury. a Western blot analysis of AQP1 expression at different reperfusion time points after IR. b Double immunofluorescence of AQP1 with different cell types of BSCB at 12 and 48 h after IR, for BSCB was suggested maximally damaged at above time points. Lines show the border of the vessels. Arrows show their co-localizations. Scale bars 100 μm. c Integrated density values of AQP1 in IR group with different reperfusion time points. After normalized against Sham group, IR-induced AQP1-upregulation increased with time. **P < 0.05 versus Sham group. d Quantification of AQP1-positive cell types of BSCB after IR (cells with yellow signals). Data are presented as mean numbers of positive cells/area/spinal section ± SEM (n = 6). **P < 0.05 versus Sham group; ## P < 0.05 versus IR group. Representative immunofluorescence and quantification showed that IR-induced AQP1 upregulation in astrocytes appeared at the early phase of BSCB dysfunction and endothelial cells emerged with the development of injury, but no identical fluorescence label of AQP1 was found in microglial cells