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Fig. 1 | BMC Neuroscience

Fig. 1

From: A non-aggressive, highly efficient, enzymatic method for dissociation of human brain-tumors and brain-tissues to viable single-cells

Fig. 1

Brain tumor (BT) dissociation to single cells using various enzymes. a Cellular viability and b dissociation cumulative grade (CG) for BTs dissociated with dispase (Disp), papain, a combination of DNase, collagenase and hyaluronidase (DCH), or mechanical dissociation only (none). See text for calculation of CG. Primary brain tumors were dissociated to single cells for 1 hour (1 h), 2 hour (2 h), or overnight (ON) at optimal enzyme concentrations—(see text). After the indicated times, the cells were triturated using a Pasteur pipette and their viability and CG was determined. Statistics: Viability of Disp or DCH dissociated-tumors to mechanically dissociated tumors (P < 0.0005 or less). CG of Disp-1 h to none-1 h and to DCH-1 h (P < 0.0001 either). CG of Disp-2 h to None-2 h and DCH-2 h (P < 0.025 either). CG of dispase ON to none-ON (P < 0.0001)

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