Fig. 4

Calbindin immunohistochemistry at 2 postnatal time points revels a reduction of Calbindin positive cells. A–A″, B–B″ P14 left and middle column sections illustrates that Calb positive cells are present throughout the cortex and hippocampus, but with a reduction in the number of labeled cells. There was a trend toward more cells in the lower layers (curved brackets) but this did not reach significance. The hippocampus at P14, shows more significant changes (B–B″; dashed circles in CA1 and CA3 stratum radiatum). In the adult animal, (right column; C, C′) there is also a clear reduction in the number of Calb positive cells in the Arx ^+/− female (C′) versus control (C). D Displays the quantification of Calbindin positive cell numbers from post-natal ages. All adult section counts are presented as a percent change from control in both the cortex and hippocampus. LV lateral ventricle, VZ ventricular zone, DG dentate gyrus, CA1 Cornus Ammonis 1, CA3 Cornus Ammonis 3. Pia pia matter, L1 cortical layer 1, L2/3 cortical layers 2 and 3, L6 cortical layer 6