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Fig. 5 | BMC Neuroscience

Fig. 5

From: Developmental interneuron subtype deficits after targeted loss of Arx

Fig. 5

Calretinin immunohistochemistry at 2 embryonic and 2 post natal time points reveals alteration of expression across the ages. The top row (E14.5, A–A″) demonstrates effect of loss of Arx on Calr staining from representative e14.5 coronal images. There is an increase in the number of cells in the region of the developing internal capsule in the Arx −/y ;Dlx5/6 CIG compared to the wildtype (bar with ends) and the Arx +/− female has an intermediate number of labeled cells. In contrast, there is a decrease in the number of scattered cells throughout the ventral pallium. Dorsally, all three genotypes have a similar appearing clear migratory stream through the MZ/CP region (arrows). E18.5 (second row, B–B″) illustrates no major differences in Calr cells either ventrally or dorsally, with accumulating cells found in the hippocampus in all three genotypes (bracket). C Presents the quantification of the two embryonic time points. All counts are presented as average ranking from rostral and caudal (and dorsal and ventral) sections at e14.5 (upper) and e18.5 (lower). Adult Ages; P14 (third row; D–D″) sections illustrate that Calr positive cells are present throughout the cortex in control mice. There is a trend toward a reduction in the number of labeled cells in mutant mice but it does not reach significance (F). In the adult animal, (bottom row; E, E′) there is also a clear reduction in the number of Calr positive cells in the Arx +/− females (E′) versus control (E). F Displays the quantification of Calr positive cell numbers from adult animals. Counts are presented as a percent change from control in both the cortex and hippocampus. LV lateral ventricle, VZ ventricular zone, BG basal ganglia, Cx developing cortex, Hip hippocampus. L1 cortical layer 1, L2/3 cortical layers 2 and 3, L6 cortical layer 6

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