Fig. 4

MCT14 is expressed in the soma of neurons and in epithelial cells. Fluorescent double IHC on paraffin sections from wild-type mouse brain with the MCT14 antibody and a panel of markers to identify the cell types expressing MCT14. The MCT14 antibody staining is seen in red, cell-specific markers are seen in green, and cell nuclei stained with DAPI are seen in blue. a Colocalization of MCT14 with the whole-neuron marker Pan neuronal cocktail aimed at soma, dendrites, axons and nuclei of neurons suggests that MCT14 is expressed in the neuronal soma in cortex. b Reduced MCT14 staining following pre-incubation of anti-MCT14 with a peptide corresponding to the antibody epitope, while Pan still stains the whole neuron. c No overlap between MCT14 and the astrocytic marker GFAP. d Overlap between MCT14 and neuronal marker MAP2 in the soma of neurons. e No colocalization of MCT14 and the pre-synaptic vesicle marker synaptophysin. f Colocalization of MCT14 and Pancytokeratin, an epithelial cell marker, indicates MCT14 expression in epithelial cells of the choroid plexus in the lateral ventricles. All images were taken at ×40 magnification on a Zeiss AxioImager, and all scale bars represent 10 µm