Decreasing the expression of PICALM reduces endocytosis and the activity of β-secretase: implications for Alzheimer’s disease

Table 1 siRNA sequences used to knock down protein expression

siRNA target	Sequence	References
Total PICALM ‘A’^a	GCAUACAAUGAAGGAAUUAdTdT	Custom-designed
Total PICALM ‘B’^a	AAGCCAAGAAUUGCUAUGAdTdT	Custom-designed
PICALM isoform 1	GCAAGUACAUGGGGAGAUCdTdT	Custom-designed
PICALM isoform 2	GUACAUGGGGAGGAUUCACdTdT	Custom-designed
CHC	UAAUCCAAUUCGAAGACCAAUdTdT	[43]
GFP	GGCUACGUCCAGGAGCGCACCdTdT	[43]

^aThe siRNA sequences for total PICALM were directed against regions of the mRNA common to isoforms 1–4

ISSN: 1471-2202