Fig. 4

The relationship between missing events and SSR complexity. a The proportion of missing quanta from indicated genotype and bouton type. Each bar represents the mean ± SEM missing intracellular spontaneous miniature potential (mEJP) as a percentage. 50 extracellular events recorded from 20 Type 1b boutons of each genotype (n = 20), as well as 50 extracellular events recorded from 20 Type 1s boutons of of the OreR and MHC::mCD8-GFP-Sh genotype (n = 20), for a total of 1000 events analyzed per data point (One-way ANOVA, Bonferroni post tests for relevant genetic comparisons, not significant n.s., ***p < 0.0001). b The percent of missing events for individual bouton recordings of Type 1b and Type 1s OreR and MHC::mCD8-GFP-Sh larvae. Each point represents the percent of missing events for an individual bouton analyzed from 50 intracellular events of 20 boutons for each genotype (n = 20). Error bars represent the mean ± SEM (One-way ANOVA, Bonferroni post test, **p < 0.001). c The relationship between percent missing events and SSR size is shown in where each point represents the mean ± SEM of percent missing events and SSR complexity (using Dlg width (μm)). The linear regression line shows a positive slope of 10.4 % missing events per micron Dlg width and with an r² value of 0.8. The slope is statistically different from zero