Fig. 2

MiR-34b-5p upregulation in kainic acid induced astrocyte apoptosis. a Primary astrocytes were treated with 50 μM, 100 μM, 150 μM or 200 μM kainic acid for 24 h followed by TUNEL staining and quantification for the percentage of TUNEL positive cells. n = 5 for each treatment group (one-way ANOVA test, F _4,20 = 70.24, ***P < 0.0001; **50 μM KA versus control, 50 μM KA versus 100 μM and 100 μM versus 150 μM P < 0.01, *** individual KA treatment versus control P < 0.0001). b Representative images of three repeated TUNEL staining experiments after 24-h kainic acid treatment; TUNEL (green), DAPI (blue); scale bar 50 μm. c–e Western blot and quantification. Cell lysate of 24 h 100 μM KA treated primary astrocytes was analysed with SDS-PAGE. Bcl-2, Bax, Pro-caspase-3, and caspase-3 were blotted. The significance difference of Bax/Bcl-2 was observed (n = 5, t ₈ = 12.93, ***P < 0.0001). f Quantitative PCR result: miR-34b-5p upregulated after 100 μM kainic acid treatment (n = 5, t ₈ = 9.775, ***P < 0.0001)