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Fig. 4 | BMC Neuroscience

Fig. 4

From: MicroRNA-34b mediates hippocampal astrocyte apoptosis in a rat model of recurrent seizures

Fig. 4

miR-34b-5p regulation of astrocyte apoptosis through mediating Bcl-2 expression level. a, b Western blot analysis for Bcl-2, Bax, pro-caspase-3, and cleaved caspase-3. Cell lysates were prepared from astrocytes treated with vehicle control, mimics negative control (m NC) or inhibitors negative control (i NC), miR-34b-5p mimics or inhibitors. c Bax/Bcl-2 protein ratio after quantitative analysis (one-way ANOVA, n = 5, F 4,20 = 123.8, ***P < 0.0001). d Western blot protein analysis for Bcl-2 and actin. Cell lysates were prepared from cultured astrocytes treated with control siRNA, scramble siRNA, and Bcl-2 siRNA. e Western blot analysis for pro-caspase-3, cleaved caspase-3, and actin. Cell lysates were prepared after astrocytes were treated with control siRNA, Bcl-2 siRNA, miR-34b-5p inhibitors and Bcl-2 siRNA together, and miR-34b-5p inhibitors alone. f Quantitative analysis for results in E (one-way ANOVA, pro-caspase-3 group, n = 5, F 3,16 = 211.6, ***P < 0.0001; Caspase-3-19KD group, n = 5, F 3,16 = 98.36, ***P < 0.0001; Caspase-3-17 KD group, F 3,16 = 158.7, ***P < 0.0001; ***on top of bars, comparing with control within same group; ***on top of lines, comparing between the individual bars the line covers, **P < 0.01, ***P < 0.0001)

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