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Fig. 5 | BMC Neuroscience

Fig. 5

From: Bone morphogenetic protein-2-mediated pain and inflammation in a rat model of posterolateral arthrodesis

Fig. 5

Implantation of ACS next to spine is sufficient for triggering numerous molecular and cellular changes in DRG and spinal nerve. a, b QPCR analysis of RNA isolated from ipsilateral and contralateral DRGs of rats 1 or 7 days (ATF-3 and CD68, respectively) after either implantation of vehicle/ACS (sponge) or the same surgical incisions but without ACS implants (no sponge) (mean ± SEM, n = 4 *p < 0.05). c Representative image of immunohistochemistry of ED1+ cells in the spinal nerve of rats with or without ACS implanted. d Cell counts of ED1+ macrophages in the DRG and spinal nerve of the same animals (mean ± SEM, n = 5 animals/group, *p < 0.05). e Heat map showing differential expression of genes in DRG at 1 and 3 dps with implantation of vehicle/ACS (sponge) in comparison to surgery alone (no sponge), as determined by bead chip analysis. Blue color intensity indicates decreasing transcript expression as compared to the mean value across all samples. Red color intensity indicates increasing transcript expression as compared to the mean value across all samples. Each column represents data from RNA extracted from one rat

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