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Table 2 Primers used for QPCR

From: Bone morphogenetic protein-2-mediated pain and inflammation in a rat model of posterolateral arthrodesis

Gene

Forward

Reverse

Amplicon

Tm (°C)

Sequence

Position

Sequence

Position

ATF-3

TGCCTGCAGAAGGAGTCAGAG

494–514

CTTCCGGCGTCCGCCCGTTCT

650–630

157

56

CD68

AATTCACCTGGACCTGCTCTC

837–857

ACGCAGAAGGCAATGAGCACC

1004–984

168

54

C-FOS

CTGTCAACACACAGGACTTTT

262–282

CGCTCTGGTCTGCGATGGGGC

410–390

149

50

CGRP

AACCTTAGAAAGCAGCCCAGGCATG

102–126

GTGGGCACAAAGTTGTCCTTCACCA

442–418

246

59

COL18A1

AGGTAAGGATCTGGGCTA

3064–3081

CCTCGTTGTCCGTCCCG

3237–3221

174

50

COX-2

CGAGGACTGGGCCATGGAGTG

746–766

ACCTCTCCACCGATGACCTGA

864–844

119

57

DYN

GCCCCAGCAGGAAGGGTGATA

211–231

CAGGGACGAAATCAGGGGGTT

371–359

157

57

GAPDH

ACCATCTTCCAGGAGCGAGA

292–311

GGCGGAGATGATGACCCT

438–421

147

56

ID1

GTGAGCAAGGTGGAGATACTG

304–321

GCTGGAACACATGCCGCCTCG

482–462

179

52

IL-1β

GACCCCAAAAGATTAAGGATT

136–156

AAAGAAGGTGCTTGGGTCCTC

335–315

200

50

MCP-1

CCAGAAACCAGCCAACTCTC

25–44

CCGACTCATTGGGATCATCT

216–197

192

53

S100A8

GTGCAGAATAAAAATACCGAA

159–179

GCTGTCTTTATGAGCTGCCAC

281–261

123

48

SMAD6

ACTGGATCTGTCCGATTCTAC

873–893

CCGGAGCTCCCAGTACGCCAC

1023–1003

151

50

SMAD7

AGAGTCTCCCCCTCCTCCTTA

755–775

AAGAAGTTGGGAATCTGAAAG

899–879

142

48

SUB P

AGAGGAAATCGGTGCCAAC

2128–2107

TGCCCATTAATCCAAAGAACTG

1456–1474

152

52

TNF-α

CGGTCCCAACAAGGAGGAGAA

323–343

AGGAGGGCGTTGGCACGCTGG

502–482

180

50

TRPV1

TACTACAAGGGCCAGACAGCA

672–692

GGGGCAGCTCACCAAAGTAGA

834–814

163

55