Fig. 3

Increment of endocytotic αSYN uptake by LRRK2-KO microglia. a Confocal immunostaining images of αSYN and Rab5 in KO and WT microglia. Scale bar 50 μm. Higher-magnification views of the boxed area show the detail of immunopositive particles. Scale bar 20 μm. b Cytofluorograms for αSYN and Rab5 immunostaining evaluated using the high magnification views of KO and WT microglia. c The αSYN/Rab5 correlation coefficient (Rcoloc) values for KO and WT microglia. Ten cells were randomly chosen per group. In all graphical representations, data are expressed as mean ± SD and were assessed by Student’s t test; *p < 0.05. The experiment was carried out three times using primary microglia isolated from independent mice, and a representative image and data are shown