Solution | Purpose or name | Composition |
---|---|---|
Bathing solution | Normal Tyrode’s solution | 136.5 mM NaCl, 5.4 mM KCl, 1.8 mM CaCl2, 0.53 mM MgCl2, 5.5 mM glucose, and 5.5 mM HEPES–NaOH buffer, pH 7.4 |
Pipette solution | For recordings of K+ current or membrane potential | 130 mM K-aspartate, 20 mM KCl, 1 mM KH2PO4, 1 mM MgCl2, 3 mM Na2ATP, 0.1 mM Na2GTP, 0.1 mM EGTA, and 5 mM HEPES–KOH buffer, pH 7.2 |
For recordings of Na+ or Ca2+ currents | 130 mM Cs-aspartate, 20 mM CsCl, 1 mM KH2PO4, 1 mM MgCl2, 3 mM Na2ATP, 0.1 mM Na2GTP, 0.1 mM EGTA, and 5 mM HEPES–CsOH buffer, pH 7.2 |