Table 1 Primer sequences for RT-PCR gene expression analysis
Gene | Sense primer (5′–3′) | Anti-sense primer (5′–3′) | Cycles | Product (bp) |
|---|---|---|---|---|
Parp1 | TCGATGGGAAAGTCCCACAC | CATTCTGAGCCTTGAGGGCC | 28 | 600 |
Parp2 | GACAATCGAGACTCTGTGAA | AGACTGGTAACCGGCCTTGA | 28 | 600 |
Gapdh | ACCACAGTCCATGCCATCAC | TCCACCACCCTGTTGCTGTA | 28 | 452 |
Cdkn1a | GTGATTGCGATGCGCTCATG | TCTCTTGCAGAAGACCAATC | 26 | 387 |
Fas | ATGCTGTGGATCTGGGCTGT | GTTTTCAGGTTGGCATGGTT | 28 | 190 |
Pidd | ATGGCTGCAGTGTTGGAGGG | CTCTGAGAGATGGTTGTGAG | 28 | 500 |
Tnfrsf10b | ATGGAGCCTCCAGGACCCAG | GAGCTCCAATCAGCAGCACT | 28 | 643 |
Pmaip1 | ATGCCCGGGAGAAAGGCGCG | GGTTACTAAATTGAAGAGCT | 28 | 308 |
Bbc3 | CCTCAGCCCTCCCTGTCACCAG | GGGTGAGGGTCGGTGTCGAT | 35 | 232 |
Perp | ATGCTGCGCTGCGGCCTGGC | GGAACAACCAATCAAGATGA | 28 | 500 |
Ccng1 | ATGATAGAAGTACTGACAAC | GGTGTCGTGAACGAGTGAAT | 24 | 500 |
Trp53 | GGAGACATTTTCAGGCTTATGG | AGAAGGGACAAAAGATGACAGG | 28 | 232 |