Fig. 3From: TDP-43 protein variants as biomarkers in amyotrophic lateral sclerosisValidation of detection phage using capture ELISA with scFvs selective for ALS TDP-43 variants. Soluble antibody fragments from 5 (ALS-TDP6, ALS-TDP13, ALS-TDP15, ALS-TDP18 and AD-TDP3) of the 23 scFvs were produced and utilized as the capture scFvs in our phage capture ELISA. a TDP-43 immunoprecipitated from ALS, FTD and healthy brain tissue (motor cortex) was the antigenic source and bound antigens were detected using TDPM1. The results were represented as a difference between the ALS and both the FTD and control groups. TDPM1 produced strong signals with all 5 scFvs. b In a similar manner, substitution of brain tissue for the immunoprecipitated protein with all five capture scFvs and TDPM1 showed positive signals with ALS relative to both FTD and healthy tissue. Bound phages were detected with an anti-M13-HRP antibody in these capture ELISAsBack to article page