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Fig. 1 | BMC Neuroscience

Fig. 1

From: Effect of Spp1 on nerve degeneration and regeneration after rat sciatic nerve injury

Fig. 1

Spp1 expression in injured rat sciatic nerve and cultured Schwann cells. a Real-time qPCR analysis of Spp1 expression in injured sciatic nerves 0, 6, 12, and 24 h as well as 1 and 2 weeks post injury. GAPDH was used to normalize the data. The average of three independent experiments is shown as mean ± SEM (*p < 0.05). b Western blot analysis of Spp1 expression levels in injured sciatic nerves 0, 6, 12, and 24 h as well as 1 and 2 weeks post injury. GAPDH levels were used as a control. c Relative protein expression levels of Spp1 in Western blot analyses. d Immunofluorescence staining of S100 and Spp1 (and their overlay) in distal sciatic nerve stumps of rats at the indicated times (14 and 28 days) and in the normal sciatic nerve. S100 was used as an SC-specific marker. Spp1 is colocalized in the plasma membrane of S100-positive Schwann cells (scale bar 50 μm). e Immunofluorescence staining of S100 (green) and Spp1 (red) (and their overlay) in cultured Schwann cells (scale bar 50 μm). Each experiment was repeated three times

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