Fig. 5

HS attenuates the expression of RBP-JK and Hes-1 in hypoxia-activated BV-2 microglia. a The immunoreactive bands of RBP-JK (56 kDa), Hes-1 (37 kDa) and β-actin (42 kDa), respectively. Bar graph b, c show the protein expression of RBP-JK and Hes-1 are significantly increased in OGD BV-2 cells compared to control group (*P < 0.05); but, it is decreased following treatment with 80 mM HS or 10 μM DAPT (#P < 0.05). The values represent the mean ± SD in triplicate. d The immunofluorescence of RBP-JK (G, J, M, P, red), lectin⁺ microglia (F, I, L, O, green) and the co-localization of RBP-JK in BV-2 microglia (H, K, N, Q). e The immunofluorescence of Hes-1 (G, J, M, P, red), lectin⁺ microglia (F, I, L, O, green) and the co-localization of Hes-1 in BV-2 microglia (H, K, N, Q). The results are aslo consistent with the western blotting. Note that the expression of RBP-JK and Hes-1 are increased in OGD-activated BV-2 cells and decreased with the treatment of 80 mM HS or 10 μm DAPT. HS hypertonic saline, DAPT N-[N-(3,5-difluorophenacetyl)-1-alanyl]-S-phenylglycine t-butyl ester, OGD oxygen glucose deprivation. NICD Notch intracellular domain, RBP-JK recombination signal sequence-binding protein J, Hes-1 Hairy-Enhancer of Split-1. Scale bars in F–Q 20 μm