Fig. 6

TNFalpha and GFAP immunostaining in the superficial lamina (lamina I and II) of dorsal horns of the spinal cord at cervical 7 segmental level. A–C Representative immunoreactivity in FRC rat for TNFalpha, GFAP, or both together. Arrow indicates same cells in each panel, showing a lack of overlap between TNFalpha and GFAP. Scale bars 50 μm, and applies to all other panels except insets. D–F Representative immunoreactivity in 12-week HRHF rat for TNFalpha, GFAP, or both together. Arrows and lettering a–c indicate cells shown enlarged in insets of each panel and depicts cells with TNFalpha and GFAP co-localization. G, H Quantification of percent area with TNFalpha and GFAP immunostaining. **p < 0.01, compared to food restricted control (FRC) rats