Fig. 2

Effects of antidepressants on levels of phospho-mTORC1 in the rat hippocampus. Rats (n = 6 animals/group) were given a daily injection of vehicle (Veh; 1 mL/kg), ESC (10 mg/kg), or PAR (10 mg/kg) for 21 days with or without restraint stress (6 h daily for 21 days). Levels of phosphorylated mTORC1 in brain homogenates from the hippocampus were detected by SDS-PAGE and Western blot analyses using anti-phospho-Ser²⁴⁴⁸-mTORC1 antibodies. A representative image and quantitative analysis normalized to the levels of total mTORC1 are shown. Results are expressed as a percentage of vehicle control and represent the mean ± standard error of the mean (SEM) of 6 animals per group. *p < 0.05 versus vehicle control; ^† p < 0.05 versus stress + vehicle