Fig. 3

Effects of antidepressants on the levels of mTORC1 downstream effectors (phospho-4E-BP-1, phospho-p70S6 K, phosphor-eIF4B, and phospho-S6) in the rat hippocampus. Rats (n = 6 animals/group) were given a daily injection of Veh (1 mL/kg), ESC (10 mg/kg), or PAR (10 mg/kg) for 21 days with or without restraint stress (6 h daily for 21 days). Levels of phosphorylated 4E-BP-1, p70S6 K, eIF4B, and S6 in brain homogenates from the hippocampus were detected by SDS-PAGE and Western blot analyses using anti-phospho-Thr^37/46-4E-BP-1 (a), anti-phospho-Thr³⁸⁹-p70S6 K (b), anti-phospho-Ser⁴²²-eIF4B (c), and anti-phospho-Ser^240/244-S6 (d) antibodies. A representative image and quantitative analysis normalized to the levels of total 4E-BP-1 (a), p70S6 K (b), eIF4B (c), and S6 (d) are shown. The results are expressed as a percentage of vehicle control and represent the mean ± SEM of 6 animals per group. **p < 0.01 versus vehicle control; ^† p < 0.05 or ^†† p < 0.01 versus stress + vehicle