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Fig. 5 | BMC Neuroscience

Fig. 5

From: Lateral cortical Cdca7 expression levels are regulated by Pax6 and influence the production of intermediate progenitors

Fig. 5

Effects of forced expression of Cdca7 in vivo. A Schematic showing the expression patterns of Pax6 (in radial glial cells), Tbr2 (in intermediate progenitor cells) and Tbr1 (in postmitotic neurons). Area in box is shown in EG. B Cdca7 expression vector pCAGGS_Cdca7 containing Cdca7 open reading frame. C Cdca7 expression plasmid was transiently transfected into HEK293 cells. Cell lysates were collected 48 h post transfection and Cdca7 expression was verified using an antibody against the HA epitope. The molecular weight of Cdca7 was approximately 43 kDa. GAPDH was used as a loading control. D Control (pCAGGS_GFP) or Cdca7 expression vector was used for in utero electroporation at E12.5 or E14.5. BrdU was injected 30 min before termination. EJ Immunofluorescence images show the expression of GFP and Cdca7 in the electroporated lateral cortex. HJ Almost all GFP+ cells co-express Cdca7 at levels obviously higher than endogenous. Outlines of GFP+ cells are overlaid on Cdca7 cells in J. White arrows indicate a cell with strong GFP but Cdca7 levels that are not obviously raised above endogenous. Red arrows indicate a cell with a low GFP level but a high Cdca7 level. Scale bars EG 200 µm; HJ 50 µm

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