Table 2 Nerve regeneration in healthy Wistar rats and in diabetic Goto-Kakizaki (GK) rats
hCNG-Ihealthy (n = 5) | CFeCNG-Ihealthy (n = 6) | hCNG-Idiabetic (n = 11) | CFeCNG-Idiabetic (n = 7) | p-values (KW) | Fisher’s methoda | ||
|---|---|---|---|---|---|---|---|
hCNG-I/CFeCNG-I | Healthy/diabetic | ||||||
Preoperative B-glucose (mmol/l) | 3.9 [3.7–4.3] | 4.0 [3.4–4.1] | 7.9 [7.1–8.7] | 8.1 [6.7–8.6] | 0.0001 | 0.96 | <0.0001 |
Regenerative Matrix Present (non-complete/complete single-strand/complete double-strand) | 1/4/0 | 2/2/2 | 8/3/0 | 0/1/6 | NA | NA | NA |
Axonal outgrowth (neurofilament present in SND) | 5/5 | 6/6 | 11/11 | 7/7 | NA | NA | NA |
ATF-3 (% of total) | |||||||
At 3 mm | 8.1 [3.0–9.1] | 8.8 [6.2–10.6] | 6.0 [4.5–10.4] | 9.5 [5.2–11.1] | 0.51 | NA | NA |
In conduit | TFO | 11.4 [6.8–12.7]d | 16.8 [15.8–22.3]b | 14.2 [10.3–22.5] | 0.29 | NA | NA |
In SND | 7.3 [6.2–16.7] | 10.2 [8.6–12.9] | 12.2 [9.4–14.3] | 6.5 [6.0–11.1] | 0.23 | NA | NA |
Cleaved caspase-3 (% of total) | |||||||
At 3 mm | 30.5 [27.4–37.7] | 31.2 [25.2–33.1] | 31.9 [28.8–46.8] | 19.1 [14.6–20.1] | 0.004 | 0.001 | 0.02 |
In conduit | TFO | TFO | 14.2 [12.5–16.1]c | 12.9 [11.1–17.6] | 0.082 | NA | NA |
In SND | 28.0 [22.1–33.5] | 28.6 [25.2–38.8] | 37.8 [27.1–41.7] | 37.4 [30.7–39.2] | 0.36 | NA | NA |
DAPI stained cells (no/mm2) | |||||||
At 3 mm | 167 [139–195] | 156 [129–185] | 184 [152–206] | 169 [151–232] | 0.59 | NA | NA |
In conduit | TFO | 3490 [3362–3980]d | 1727 [1412–2921] | 3198 [1991–3635] | 0.21 | NA | NA |
In SND | 2823 [2201–3234] | 2542 [2083–3036] | 3071 [2445–3454] | 2732 [2330–3733] | 0.60 | NA | NA |