Fig. 2

CLCA4L-mediated whole cell currents from HEK293 transfected with pIRES-2 EGFP-CLCA4L. a Cells under phase contrast; a patch clamp electrode is attached to one of the cells (left). Fluorescence image of the same field, with two cells displaying GFP-fluorescence, indicative of transfection (right). Calibration Bar: 10 µm. b Left, whole cell currents of a pIRES GFP⁺ CLCA4L-expressing cell under 0.9 µM Ca²⁺ internal solution, induced by voltage ramps in the absence (control) or presence of 30 µM external niflumic acid, and after washout; control and wash traces superpose (N = 6). Center a cell under 0.06 µM Ca²⁺ internal solution (N = 6). Right a pIRES GFP⁺ CLCA4L⁻ cell under 0.9 µM Ca²⁺ internal solution (N = 8); in all cases, \({\text{Cl}}_{\text{o}}^{ - } /{\text{Cl}}_{\text{I}}^{ - }\): 110/110. c Left whole cell currents of CLCA4L-HEK293 transfected cell induced by the tail current protocol depicted at the bottom left. Center under 30 µM niflumic acid (N = 6 both). Right I–V relationship. d Left currents under \({\text{Cl}}_{\text{o}}^{ - } /{\text{Cl}}_{\text{i}}^{ - }\): 110/110. Center \({\text{Cl}}_{\text{o}}^{ - } /{\text{Cl}}_{\text{I}}^{ - }\): 10/110. Right I–V relationship (N = 6, both). e Left currents under \({\text{Cl}}_{\text{o}}^{ - } /{\text{Cl}}_{\text{i}}^{ - }\): 110/110. Center under \({\text{SCN}}_{\text{o}}^{ - } /{\text{Cl}}_{\text{i}}^{ - }\) (110/110) (N = 6; see “Methods”). Right I–V relationship. Cells recorded in all experiments in the figure were representative