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Fig. 3 | BMC Neuroscience

Fig. 3

From: The loss-of-function disease-mutation G301R in the Na+/K+-ATPase α2 isoform decreases lesion volume and improves functional outcome after acute spinal cord injury in mice

Fig. 3

Changes in α1, α2, and α3 protein levels after SCI. a α1, α2, and α3 isoform levels in naïve conditions and 3 days after SCI were evaluated in spinal cord lysates from α +/+2 and α +/G301R2 mice by Western blot analysis. Data are normalized to β-actin protein expression. Representative experiments are shown. bd Western blot quantification showed a significant increase in α1 isoform levels 3 days after SCI, with no difference between genotypes (two-way ANOVA: time ****p < 0.0001, F1,12 = 35.06, **p < 0.01) (b), a significant decrease in α2 isoform levels 3 days after SCI, with significantly reduced levels in α +/G301R2 compared to α +/+2 mice (two-way ANOVA: time ***p < 0.001, F1,20 = 16.88; genotype ****p < 0.0001, F1,20 = 26.67) (c), and a significant decrease in α3 isoform levels 3 days after SCI, with no difference between genotypes (two-way ANOVA: time ****p < 0.0001, F1,12 = 53.06) (d). Results, expressed as fold change in relation to naïve α +/+2 mice, are the mean ± SEM of 5 mice/group. ***p < 0.001, **p < 0.01, *p < 0.05, Bonferroni’s post hoc

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