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Table 2 Intra and inter-run accuracy and precision of the method (n = 5)

From: A convenient UHPLC-MS/MS method for routine monitoring of plasma and brain levels of nicotine and cotinine as a tool to validate newly developed preclinical smoking model in mouse

Concentration (ng/mL)

Intra-run

Inter-run

Accuracy

R.S.D. (%)

Accuracy

R.S.D. (%)

Plasma

 Nicotine

  LLOQ

3

101.49

7.50

107.06

9.53

  LQC

9

93.36

5.02

98.92

9.33

  MQC

75

89.67

3.55

89.35

4.64

  HQC

150

92.48

5.90

98.61

11.30

 Cotinine

  LLOQ

3

84.34

3.7

94.29

10.99

  LQC

9

105.98

6.93

100.25

14.40

  MQC

225

90.55

1.24

89.96

6.97

  HQC

450

97.89

9.5

98.31

8.13

Brain

 Nicotine

  LLOQ

3

104.26

4.36

102.59

14.48

  LQC

9

111.01

4.93

107.28

5.65

  MQC

40

114.45

7.36

109.44

9.10

  HQC

80

113.46

6.91

110.70

7.51

 Cotinine

  LLOQ

3

96.66

8.59

98.55

8.01

  LQC

9

107.36

6.56

106.23

5.46

  MQC

40

106.64

9.68

109.61

8.20

  HQC

80

112.52

1.26

113.21

1.49