PeakCaller: an automated graphical interface for the quantification of intracellular calcium obtained by high-content screening

Table 1 Comparison of software packages that measure intracellular calcium activity

Software package	Major features of calcium dynamics	References
SIMA	Amplitude Time of onset Area under the curve Frequency Activation and decay time	[19, 20]
FluoroSNNAP	Amplitude Functional connectivity Synchronization analysis Correlation coefficient Frequency Activation and decay time Wavelet-based detection	[15]
NeuroCa	Amplitude Frequency Background correction Analysis of ~ 1000 cells Network dynamics	[17]
PeakCaller	Frequency Synchronization index Correlation coefficient Event rise and fall time
MATLAB dynamics	Amplitude Frequency Time of onset after stimulus Area under the curve Response duration Full width half max Decay time constant	[16]
MATLAB script	Amplitude Frequency Automated detection of single cells in dense cultures Background correction	[18]

ISSN: 1471-2202