Fig. 3
![Fig. 3](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12868-017-0392-x/MediaObjects/12868_2017_392_Fig3_HTML.gif)
Netrin-1-induced axon branching in mouse E16 cortical neurons. Phase contrast images shown are representative of E16 neurons under basal conditions (a) and after 4 h netrin-1 stimulation (250 ng/mL) in the absence (b) or presence (c) of an anti-DCC function-blocking antibody (1 μg/mL; applied 30 min before the addition of netrin-1). Arrows indicate branch points (with branches longer than 12 μm and/or lamellipodium-tipped) along the primary axon. In b, an inset showing a DIC image provides a magnified view of the branch points indicated by the arrows No. 1 and No. 2. Scale bars, 20 μm