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Fig. 2 | BMC Neuroscience

Fig. 2

From: Activity-dependent formation of a vesicular inhibitory amino acid transporter gradient in the superior olivary complex of NMRI mice

Fig. 2

Developmental gradient of VIAAT immunoreactivity in the SOC of NMRI mice. A: Immunohistochemistry against VIAAT in the SOC at P30, P16 and P8 in NMRI mice. A strong gradient in immunoreactivity was found in the LSO and SPN at P30 with higher signal in lateral regions (a, a′, a″). This gradient was also visible at the LSO at P16, yet less pronounced (b, b′). No gradient was evident at P8 (c, c′). There was no gradient in other SOC nuclei outside the LSO and SPN (a, b). Dorsal is up, lateral to the right. Scale bars = 100 µm. B, C: Quantification of VIAAT-immunoreactivity in the SPN at P30 (B) and in the LSO at P30, P16 and P8 (C). Gray values were determined at medial and lateral regions of the respective nucleus. Bars only depict lateral values in % compared to medial. Medial values were set to 100% (dashed line). Gray values in the lateral limb of the LSO and SPN were significantly higher at P30 compared to the medial one. Significant higher immunoreactivity in the lateral LSO was already established at P16. As a test for statistical significance, Mann–Whitney U test was used. 14–15 ROIs were analyzed per animal and three animals per age. **p ≤ 0.05; ***p ≤ 0.001, ns not significant

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