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Fig. 3 | BMC Neuroscience

Fig. 3

From: Altered phosphorylation, electrophysiology, and behavior on attenuation of PDE4B action in hippocampus

Fig. 3

Increased pCREB, pERK and neurogenesis in PDE4B1-D564A transgenic mice. All data in the bar graphs was obtained using ImageJ, where an increased signal is indicated by a lower intensity. All data are mean ± SE. Asterisks (*) show data from the transgenic mice that is statistically different from that obtained from wild-type mice. a The PDE4B1-D564A transgene increases pCREB and pERK1/2 in prepiriform cortex and lateral olfactory tract. (i) Nissl staining. Boxes indicate regions shown in (ii); PPC: Prepiriform cortex; Lot: lateral olfactory tract. The arrow indicates the border of the piriform cortex. (ii) pCREB and pERK1/2 expression in PPC and Lot. Upper 4 panels: Expression of CREB and pCREB in prepiriform cortex in WT and TG mice, respectively. Lower 4 panels: Expression of ERK1/2 and pERK1/2 in the lateral olfactory tract of WT and TG mice, respectively. Increased pERK1/2 immunoreactivity was evident as a larger and more intense region of staining. b The PDE4B1-D564A transgene increases pERK1/2 in hippocampus. (i) Top pair: total ERK1/2 expression is not detectably different; ERK1/2 expression was highest near the surface of the gyrus. Second pair: pERK1/2 expression is increased in transgenic (TG), as opposed to wild-type (WT) mice in the mossy fibers projecting from the dentate gyrus. Third pair, wider field: pERK1/2 expression is increased in transgenic (TG), as opposed to wild-type (WT) mice in the mossy fibers projecting from the dentate gyrus. (ii) Enlarged views of pERK1/2 expression in dentate gyrus, with areas used for quantitation of pERK1/2 expression outlined in red. The hilus and mossy fibers are shown. (iii) Quantitation of pERK1/2 immunoreactivity in hilus and mossy fibers, as determined by ImageJ

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