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Table 2 Reference gene expression stability values in the tissues and cells based on several programs

From: Evaluation of reference genes for gene expression studies in mouse and N2a cell ischemic stroke models using quantitative real-time PCR

Samples

Rank

geNorm

NormFinder

BestKeeper

ΔCp

General ranking

  

Genes

M

Genes

Stability index

Genes

SD

Genes

Average SD

Genes

Tissues

1

HPRT

0.394

18S

0.193

HPRT

0.250

18S

0.568

HPRT

2

Cyclophilin

0.394

HPRT

0.206

Cyclophilin

0.340

HPRT

0.572

18 s

3

18S

0.512

Sdha

0.223

18S

0.370

Sdha

0.607

Cyclophilin

4

Sdha

0.558

β-actin

0.223

β-actin

0.460

β-actin

0.623

Sdha

5

β-actin

0.583

Cyclophilin

0.230

Sdha

0.520

Cyclophilin

0.626

actin

6

GAPDH

0.611

GAPDH

0.247

GAPDH

0.550

GAPDH

0.662

GAPDH

Cells

1

β-actin

0.618

β-actin

0.228

18S

0.400

β-actin

0.671

β-actin

2

Cyclophilin

0.618

Cyclophilin

0.310

Cyclophilin

0.410

Cyclophilin

0.734

Cyclophilin

3

GAPDH

0.670

GAPDH

0.367

GAPDH

0.480

GAPDH

0.811

GAPDH

4

18S

0.695

HPRT

0.419

β-actin

0.510

HPRT

0.84

18S

5

HPRT

0.753

18S

0.439

HPRT

0.640

18S

0.877

HPRT

6

Sdha

0.810

Sdha

0.451

Sdha

0.880

Sdha

0.921

Sdha