Fig. 6

Culture media modifies pericyte migration following a scratch wound injury. Human brain pericytes were plated at a low density and allowed to proliferate in either DMEM/F12 with 10% FBS or Pericyte Medium for 7–14 days. The resulting confluent pericyte monolayer was scratched down the centre of the well using a sterile 200 µL pipette tip and wells were washed twice with PBS to remove detached cells. An equal number of wells were left unscratched. Pericytes were incubated for a further 48 h to allow migration into the scratch wound to occur. Cells were fixed and stained with Coomassie blue and imaged by bright field microscopy. Consistency of scratches between wells can be observed (a). A magnified image of a representative well shows the difference in pericyte migration (b). The gap area, defined as the percentage of each site devoid of Coomassie staining, was determined by automated image analysis (c). Data are displayed as mean ± SEM of three independent experiments. *p < 0.05; ***p < 0.001 (Two-way ANOVA with Bonferroni post-test). Scale bar = 50 µm