Fig. 11

Qualitative MRI and histological evaluation of the lateral corticospinal tract degeneration in the spinal cord from an ALS patient. MRI and histology was assessed at the same plane (a). The structural MRI (b) shows lateral corticospinal tract hyperintensity (yellow arrow and circle) compared to normal appearing white matter region (red circle). Images c, d represent low magnification snapshots of the spinal cord stained with haematoxylin and eosin and PLP, respectively. Images in the bottom row demonstrate qualitative comparison between normal appearing white matter in red boxes and lateral corticospinal tract in yellow box inserts at ×20 objective magnification for CD68 (e), ferritin (f), SMI-312 (g) and PLP (h) stains. Quantitative MRI and histology analysis outputs for these regions are presented in Table 4. Scale bar for c, d = 1 mm. Scale bar for figures e–h and corresponding inserts = 100 µm