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Table 4 Quantitative MRI and histology evaluation of the lateral corticospinal tract of the spinal cord in ALS

From: Dissecting the pathobiology of altered MRI signal in amyotrophic lateral sclerosis: A post mortem whole brain sampling strategy for the integration of ultra-high-field MRI and quantitative neuropathology

Regions

MRI

Histology

FA

MD (×10−5 mm2/s)

T1 (ms)

T2 (ms)

PLP

SMI-312

CD68

Ferritin

Lateral Corticospinal Tract

0.17 ± 0.03

11.23 ± 1.19

714.8 ± 31.4

34.2 ± 2.11

0.379

0.356

0.044

0.061

Normal appearing white matter

0.19 ± 0.07

7.95 ± 2.52

672.1 ± 23.6

30.9 ± 1.31

0.696

0.606

0.016

0.036

P value

< 0.001

< 0.001

< 0.001

< 0.001

NA

NA

NA

NA

  1. Quantitative MRI and histological data from the lateral corticospinal tract of the spinal cord was compared to the normal appearing white matter region as outlined in Fig. 11. Magnetic resonance imaging data were quantified per voxel in the regions of interest with two-tailed t test and reported as mean ± standard deviation. The regions of interest within the lateral corticospinal tract and normal appearing white matter were generated from structural MR image and applied to each modality that were analysed. The MR data were calculated in 276 voxels with 100 mm2 cross sectional area in the lateral corticospinal tract and in 269 voxels with 97.6 mm2 cross sectional area in the normal appearing white matter. On the corresponding histology plane, a single section was stained for each representative microstructural marker and the stained area fraction (area of positive staining over total analysis area) was calculated