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Fig. 4 | BMC Neuroscience

Fig. 4

From: 27-Hydroxycholesterol increases α-synuclein protein levels through proteasomal inhibition in human dopaminergic neurons

Fig. 4

The LXR agonist, GW3965, and the LXR antagonist, ECHS, do not affect α-synuclein protein or mRNA levels. a Lactate Dehydrogenase assay shows that 27-OHC, GW3965, ECHS, and 27-OHC + ECHS do not elicit significant cell death relative to control untreated cells. Representative western blot (b) and optical density (c) of ABCA1 expressed as fold change over β-Actin. 27-OHC and GW3965 both significantly increase the amount of ABCA1 protein levels. ECHS does not significantly alter the protein levels of ABCA1 while 27-OHC + ECHS significantly increases the amount of ABCA1. Representative western blot (d) and optical density (e) of α-syn. Western blots expressed as fold change over β-Actin. 27-OHC significantly increased the amount of α-syn protein levels while the LXR agonist GW3965 and LXR antagonist ECHS had no effects on α-syn protein levels. f Real-time rt-PCR shows that 27-OHC, GW3965, ECHS, and 27-OHC + ECHS do not significantly affect SNCA mRNA levels. Data are expressed as individual values with mean ± SEM and includes determinations made in one experiment with (n = 3) and three technical replicates for LDH assay, two separate experiments with (n = 3) and technical replicates for western blots, and one experiment with (n = 3) and three technical replicates for PCR. *p < 0.05, **p < 0.01, ***p < 0.001 versus control

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