Fig.Ā 4From: A method for estimating relative changes in the synaptic density in Drosophila central nervous systemConfocal and Super-resolution imaging of Bruchpilot and Bungarotoxin staining in the whole mount and squash preparations. A Whole-mount preparation and B squash preparation of abdominal neuromere of Drosophila 3rd larval instar VNC showing Bruchpilot (red) and Ī±-Bungarotoxin staining (green). The images were collected from the same specimen sequentially using confocal (aāaā³), and STED (bābā³, cācā³, dādā³). The object-maps of synapses or synaptic bulbs, as shown in aāaā³ and bābā³, respectively, were generated from the same regions using 3D object counter plugin of FijiĀ®. Magnification Ć93 glycerol objective, N.A.ā=ā1.3; Scale bars: 5Ā Āµm. aā², bā², cā², dā² are enlarged view of region shown in box in a, b, c, d while aā³, bā³, cā³, dā³ are enlarged view of region shown in box in aā², bā², cā², dā². Arrows in A point to a single synaptic bulb shown in whole-mount while arrows in B point to a single synaptic bulb shown in squash preparation. The images presented here are similar to the observations made in 3 such independent VNC preparations. In each preparation, the results are consistent amongst the A4āA6 abdominal hemisegments. All panels in a row are presented in the same scale as shown in one of the panels in each rowBack to article page