Skip to main content
Fig.Ā 4 | BMC Neuroscience

Fig.Ā 4

From: A method for estimating relative changes in the synaptic density in Drosophila central nervous system

Fig.Ā 4

Confocal and Super-resolution imaging of Bruchpilot and Bungarotoxin staining in the whole mount and squash preparations. A Whole-mount preparation and B squash preparation of abdominal neuromere of Drosophila 3rd larval instar VNC showing Bruchpilot (red) and Ī±-Bungarotoxin staining (green). The images were collected from the same specimen sequentially using confocal (aā€“aā€³), and STED (bā€“bā€³, cā€“cā€³, dā€“dā€³). The object-maps of synapses or synaptic bulbs, as shown in aā€“aā€³ and bā€“bā€³, respectively, were generated from the same regions using 3D object counter plugin of FijiĀ®. Magnification Ɨ93 glycerol objective, N.A.ā€‰=ā€‰1.3; Scale bars: 5Ā Āµm. aā€², bā€², cā€², dā€² are enlarged view of region shown in box in a, b, c, d while aā€³, bā€³, cā€³, dā€³ are enlarged view of region shown in box in aā€², bā€², cā€², dā€². Arrows in A point to a single synaptic bulb shown in whole-mount while arrows in B point to a single synaptic bulb shown in squash preparation. The images presented here are similar to the observations made in 3 such independent VNC preparations. In each preparation, the results are consistent amongst the A4ā€“A6 abdominal hemisegments. All panels in a row are presented in the same scale as shown in one of the panels in each row

Back to article page