Fig.Ā 5From: A method for estimating relative changes in the synaptic density in Drosophila central nervous systemEstimation of the volume occupied by synaptic contacts in whole mount and squash preparations of VNC. a Whole-mount and b squash preparation of A6 abdominal segments of 90Ā h AEL VNCs from cha-Gal4, UAS-GFP-ChAT stock were stained with the Bruchpilot antibody. Images are represented in a pseudocolor scheme. Magnification Ć40 oil objective, N.A.ā=ā1.3; Scale bars: 10Ā Āµm. c, d Box plots depict quantifications of synaptic contacts before and after applying the size filter. Pairwise comparisons were made using one-way ANOVA and Bonferroniās test (***indicates pāā¤ā0.001). The pictures presented in a, b are representatives of the sample sizes, nā=ā20 for the whole-mount, and nā=ā30 for the squash preparations. The same set was used for obtaining the box plotsBack to article page