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Fig.Ā 5 | BMC Neuroscience

Fig.Ā 5

From: A method for estimating relative changes in the synaptic density in Drosophila central nervous system

Fig.Ā 5

Estimation of the volume occupied by synaptic contacts in whole mount and squash preparations of VNC. a Whole-mount and b squash preparation of A6 abdominal segments of 90Ā h AEL VNCs from cha-Gal4, UAS-GFP-ChAT stock were stained with the Bruchpilot antibody. Images are represented in a pseudocolor scheme. Magnification Ɨ40 oil objective, N.A.ā€‰=ā€‰1.3; Scale bars: 10Ā Āµm. c, d Box plots depict quantifications of synaptic contacts before and after applying the size filter. Pairwise comparisons were made using one-way ANOVA and Bonferroniā€™s test (***indicates pā€‰ā‰¤ā€‰0.001). The pictures presented in a, b are representatives of the sample sizes, nā€‰=ā€‰20 for the whole-mount, and nā€‰=ā€‰30 for the squash preparations. The same set was used for obtaining the box plots

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