Fig. 5

Gene expression changes in photoreceptors between day one and six represent developmental transitions. a Volcano plot showing the differential gene expression profiles in the control (dark-treated) day one versus day six photoreceptors. Fold change was plotted as log₂(fold change) for each gene relative to its false discovery rate (−log₂[FDR]). Genes with significantly differential expression (FDR < 0.05) are highlighted in red or blue, and GFP is shown in green for comparison. b Gene set analysis barcode plot overlaying RNA-seq data from day one versus day six photoreceptors with age-regulated genes in photoreceptors between day 10 and 40. Day one versus day six data are shown as a shaded rectangle with genes horizontally ranked by moderated t-statistic, upregulated genes shaded in pink, and downregulated genes shaded in blue. Previously described age-regulated genes are overlaid as red (age-upregulated) or blue (age-downregulated) bars. Red and blue traces above and below the barcode represent relative enrichment. FDR values represent overlap in the same direction using the roast method; ns not significant