Fig. 6

Cluster anteriolateral to the lateral calyx (alLCA). a Schematic drawing of the medial level of one hemisphere. AmFoxP neurons are indicated by small empty circles within the boxed area which indicates the localization of panels (b–j). b Confocal image (2 µm confocal section) of a pupal brain stained with the AmFoxP^42kDa antiserum (white label). c–j dISH stainings showing AmFoxP transcript (magenta), neurotransmitter transcript (cyan) and DAPI-stained nuclei (blue) on 12 µm cryosections. Rectangular insets show magnified boxed area with co-localization of the two probes. c–f Adjacent cryosections of a 15 days old individual from the ‘unmanipulated’ group. g Cryosections of a newly emerged individual. h Cryosection of an 11 days old individual from the ‘honeycomb’ group. i Cryosections of a 31 days old individual of the ‘incubator’ group. j Cryosection of a 50 days old individual of the ‘control’ group. For explanations of pictograms see Table 1, for anatomical abbreviations see Table 5. Scale bars as indicated. Scale bar in c as orientation for the panels (d–j)