Fig. 8

Cluster ventral to the medial calyx (vMCA) and group dorsal to the central complex (dCX). a Honeybee brain schematic drawing focuses on the posterior brain. AmFoxP neurons are depicted as small empty circles. Rectangle indicates localization of panels (b–l). b Confocal image (2 µm confocal section) of a pupal brain stained with the AmFoxP^42kDa antiserum (white label). c–l dISH stainings show AmFoxP transcript (magenta), neurotransmitter transcript (cyan) and DAPI-stained nuclei (blue) on 12 µm cryosections. Arrowheads point to neurons that co-express AmFoxP and a neurotransmitter. c Cryosection of a newly emerged individual. d Cryosection of a 15 days old individual from the ‘honeycomb’ group. e–h Adjacent cryosection of a 27 days old individual from the ‘incubator’ group. i–l Cryosection of a 50 days old individual from the ‘control’ group. For explanations of pictograms see Table 1, for anatomical abbreviations see Table 5. Scale bar b: 100 µm, as orientation for all other panels