Fig. 4
CoCl2 exposure reduces mTOR signaling while rapamycin is unable to further alter pathway expression profile. a Rapamycin treatment does not restore mTOR protein expression profile during CoCl2 exposure. Cells were treated and analyzed as in Fig. 2a via Western blot. Shown is the average relative protein expression of mTOR, p-mTOR, and pS6 in each group when normalized to β-actin protein expression. #p < 0.05 versus control; ##p < 0.01 versus control; ###p < 0.001 versus control. b Representative images of at least three separate blots (cells treated as in A) showing expression of the indicated mTOR signaling proteins along with loading control β-actin. c Ratio of p-mTOR and pS6 to total mTOR levels. Cells were treated and analyzed as in A via Western blot. Shown are the average ratios of p-mTOR and pS6 in each group to total mTOR levels. #p < 0.05 versus control; ##p < 0.01 versus control