Group | N | Label | Condition | Level | Survival post-labeling | Survival post injury |
---|
1 | 10 | FG | TXN | T10 | 10Â days | 3Â days |
2 | 10* | FG | TXN | T2 | 10Â days | 3Â days |
3 | 10* | FG | Control | No injury | 10 days | – |
- Animal experimental treatment groups. Female Fischer 344 rats were divided among 3 groups. Using the indicated tracer, Fluorogold (FG) PS neurons were labeled via bilateral injections of FG into the lumbosacral enlargement prior to axotomy. The animals were allowed to recover for 1Â week, allowing the FG adequate time to retrogradely label PS neurons. Following 1-week tracer transport time, animal is groups 2 and 3 then received a complete spinal transection (TXN) at the indicated spinal level, and were then allowed to recover for 3-days, at which point the spinal tissue was harvested for laser capture microdissection and gene expression analysis
- *Three animals in the T2 SCI group and 1 in the uninjured control group were euthanized prior to the planned date due to health complications