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Fig. 3 | BMC Neuroscience

Fig. 3

From: Effect of photobiomodulation therapy on neuronal injuries by ouabain: the regulation of Na, K-ATPase; Src; and mitogen-activated protein kinase signaling pathway

Fig. 3

Irradiation was performed 30 min after 5 mM ouabain treatment. Cells were incubated for 24, 48, or 72 h with ouabain, and cell morphological changes were observed using an inverted microscope and photographed at 200 × magnification (a). The viability of HCN-2 cells with ouabain after irradiation with various energy doses was measured by MTT assay. Cell survival increased from 64.7 to 81.8% at 3.12 J/cm2 irradiation. However, there was a decrease in cell viability at 9.36 J/cm2 irradiation (b). Every assay was performed three times, and the results are expressed as the mean ± SD. *p < 0.1 and ***p < 0.001

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