Fig. 1From: Live calcium imaging of Aedes aegypti neuronal tissues reveals differential importance of chemosensory systems for life-history-specific foraging strategiesRNAseq expression, schematic representation of the GCaMP6s construct and larval fluorescence. Log2 (RPKM) expression values for the promoter, AAEL003877 (PUb) was plotted across development. Samples include, from left to right: testes; male carcasses (lacking testes); carcasses of females prior to blood feeding (NBF); female carcasses 12 h, 24 h, 36 h, 48 h, and 72 h post blood meal; ovaries from NBF females and at 12 h, 24 h, 36 h, 48 h, and 72 h post ecdysis; embryos from 0–2 h through 72–76 h; whole larvae from 1st instar, 2nd instar, 3rd instar and 4th instar; male pupae; and female pupae. A genome browser snapshot was with the Y axis showing expression level based on raw read counts of fourth instar larvae (a). A schematic representation of the piggyBac-mediated GCaMP6s construct. GCaMP6s is driven by AAEL003877(PUb)(blue) while dsRed by OpIE-2, the latter serving as a transgenic marker (b). Larval bright field images (left) and corresponding fluorescent images (right) show robust GFP transients throughout the whole body and DsRed fluorescence in the abdomen. +/+ represents wild-type larva. GCaMP6s/+/+ represents transgenic GCaMP6s larvae (c)Back to article page