Table 1 Comparison of internal structure textures of the median nerve sections following different staining methods
From: An enhanced staining method K-B-2R staining for three-dimensional nerve reconstruction
Group | Karnovsky–Roots staining | K-B staining | K-B-2R staining |
|---|---|---|---|
Nerve fascicle texture | Aggregation region of diffuse spots | Pale blue circle-like region | Reddish circle-like region |
Nerve fascicle edge | No | Indistinct | Clear |
Space among nerve fascicles | Slight non-specific staining | Intermittent non-specific staining | Even staining |
Myelin sheath | No | No | Clear |
Motor nerve fiber | Indistinct | Indistinct | Explicit |
Sensory nerve fiber | Indistinct | Indistinct | Explicit |
Relationship between myelin sheath and typical spots of nerve fibers | – | – | Explicit |
Acetylcholinesterase-positive site | Brownish-yellow | Blue black | Black |
Transitional region among spots | Occasional non-specific staining | Even pale blue | Reddish |